Optimization for cellulase production by Aspergillus Niger: Fungal cellulase optimization by Aspergillus Niger
Microorganisms bring about most of the cellulose degradation occurring in nature. In the present study, optimization for cellulase production by Aspergillus niger was investigated by using paddy straw as substrate.Cellulase enzyme was produced by the fungus extracellularly. Optimization of cellulase production was done by using various physical (Temperature, pH, Salinity and Incubation time) and chemical parameters (Carbon sources and nitrogen sources) which could influence the enzyme activity. Cellulase production was maximum at the temperature 20°C and minimum at 40ºC. The optimal pH for the cellulase production was observed maximum in 6.0 and minimum in 7.0. Cellulase production was maximum at 48 hours and minimum at 24 hours. Cellulase production was maximum with when fructose was used as a carbon source and minimum with sucrose. Cellulase production was maximum when Malt extract was used as a nitrogen source and minimum with yeast extract. The cellulase enzyme extract was partially purified and its protein fraction was stained with its molecular weight determination by using SDS-PAGE. It revealed two protein bands with the molecular weights of about 83 kD and 50kD.
yazar | Saranraj J. P. |
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Boyutlar ve boyutlar | 15 x 0,5 x 22 cm |
Tarafından yayınlandı | 12 Nisan 2012 |
15 x 0,3 x 22 cm BrownTrout Publisher 1 Ocak 2018 15 x 0,5 x 22 cm 1 Haziran 2018 15 x 0,6 x 22 cm Flower Love Press 21,6 x 0,6 x 27,9 cm Dazzle Book Press 15 x 0,4 x 22 cm U.S. Department of the Interior 30 Eylül 2020 1 Ocak 2017 Collectif Kolektif 15 x 0,7 x 22 cm 28 Şubat 2018 1 Ocak 2016
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Sürüm ayrıntıları
yazar | Saranraj J. P. Stella D. |
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isbn 10 | 3848490552 |
isbn 13 | 978-3848490554 |
Yayımcı | LAP LAMBERT Academic Publishing |
Dilim | İngilizce |
Boyutlar ve boyutlar | 15 x 0,5 x 22 cm |
Tarafından yayınlandı Optimization for cellulase production by Aspergillus Niger: Fungal cellulase optimization by Aspergillus Niger | 12 Nisan 2012 |
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